SHOCK SOCIETY
TWENTY-FIFTH ANNUAL
CONFERENCE ON SHOCK
Big Sky Resort
Big Sky, MT
June 8-11, 2002
8:00
AM - 2:00 PM COUNCIL MEETING
Shoshone Boardroom
12:00 - 6:00 PM Registration
Firehole
1:30 - 1:35 PM Welcome and Introduction
Missouri Ballroom H.
Hank Simms, MD, North Shore-Long Island Jewish Medical
Center,
New York University School of Medicine, Manhasset, NY
1:35 -2:30 PM
PLENARY I: Insights Into the Mechanisms of Burn, Sepsis Missouri Ballroom and
Shock Induced Organ Injury, Papers 1-4
Moderators:
Kevin Tracey, MD, North
Shore-Long Island Jewish Medical Center, New York University School of
Medicine, Manhasset, NY and Antonio DeMaio, PhD, Johns Hopkins University, Baltimore, MD
1:35 – 1:45 PM MIP-2 Is
Involved in Hepatocyte Proliferation After 70% Hepatectomy Via a STAT-3
Mediated Pathway, Paper 1
Xiaodan
Ren, MD, University of Michigan, Ann Arbor, MI
1:45 – 1:55 PM Anti-HMGB1
Antibodies Protect Against Sepsis Lethality In
Mice,
Paper 2
Huan Yang, PhD, North
Shore-Long Island Jewish Medical Center, New York University School of
Medicine, Manhasset, NY
1:55 – 2:05 PM Burn Injury Induces An Inhibitory Signal In the Lung SMAD
Pathway,
Paper 3
Tam Pham, MD, Shriners
Hospital for Children, University of California at Davis, Sacramento, CA
2:05 – 2:15 PM Macrophage
Migration Inhibitory Factor (MIF) is a Novel
Cardiac-Derived
Myocardial Depressant, Paper
4
Leslie Garner, MD, UT
Southwestern Medical Center, Dallas, TX
2:15 - 2:30 PM Questions/Answers with Plenary Panel
2:30 - 5:30 PM
WORKSHOP I: Current Status of Federal Funding in
Missouri Ballroom Shock/Trauma Research
Moderator:
Scott Somers, PhD, NIH, Program Administrator,
NIGMS, Bethesda, MD
This workshop will focus on multiple aspects of federal
funding in shock/trauma research.
Experts from the NIH, DOD and DARPA will discuss areas of interest to
these organizations, funding mechanisms available and the process for securing
funding. In addition, speakers will
address the important topic of future areas of investigation deemed to be
significant by these funding organizations.
2:30 – 3:30 PM NIH Michael Rogers, PhD, Director Division of
Pharmacology,
Physiology & Biological Chemistry,
NIGMS
3:30 – 4:30 PM DOD Col. Robert Vandre, (USAMRMC);
Jeannine Madje-
Contrell,
PhD, (ONR) Arlington, VA
4:30 – 5:30 PM DARPA Joseph T. Bielitzki, MS, DVM,
Director for Metabolic Engineering, Arlington, VA
7:30
- 8:30 PM Presidential Keynote Address:
Missouri Ballroom “Double Bubble Toil and Trouble” The Caldron
of Research, Academia, and the
Private Sector
Carol
Wells, PhD, University
of Minnesota, Minneapolis, MN
8:30
- 9:30 PM WELCOME RECEPTION
Lone Peak Cafe
SUNDAY, JUNE 9, 2002
7:00
– 8:00 AM Continental
Breakfast
Lone Peak Café
7:00
– 8:30 AM Editorial
Board Breakfast
Talus
7:30
- 10:00 AM POSTER SESSION I, Papers 5-60
Mammoth Room
Adhesion
Molecules, Papers 5-6
Animal
Models, Papers 7-11
Burn/Trauma,
Papers 12-22
Cell
Signaling, Papers 23-34
Cellular/Molecular,
Papers 35-41
Clinical
Applications, Papers 42-45
Cytokines,
Papers 46-60
10:00 – 12:00 Symposium
I: Critical Receptor/Ligand
Interactions in the Missouri Ballroom Pathogenesis of Shock
Moderator: Richard Turnage, MD, University of Texas, Southwestern
Medical Center, Dallas, TX
Recent advances in molecular and cellular biology have identified key receptor/ligand interactions that contribute to the physiologic derangements seen during shock. The aims of this symposium are to review several of these receptors and their ligands, discuss the downstream pathways and provide pre-clinical or clinical evidence of the role of these receptor/ligand interactions in the pathogenesis of shock. In this symposium, speakers will also address the issue of the biological redundancy of these pathways.
10:00 – 10:20 AM Lipopolysaccharide
and Toll-like Receptors. Hobart
Harris, MD, UCSF, San Francisco, CA
10:20 – 10:40 AM Endothelin and
Its Receptors. Mark Clemens, PhD,
University
of North Carolina, Charlotte, NC
10:40 – 11:00
AM Colony-Stimulating
Factor and their Receptors. Alicia
Mohr,
MD, UMDNJ, Newark, NJ
11:00 – 11:20
AM Complement
and Receptors. Francis D. Moore
Jr., MD,
Brigham & Women’s Hospital, Harvard
Medical School,
Boston, MA
11:20 – 11:40
AM Novel
Cytokines and Their Receptors. Ori
Rotstein, MD,
University of Toronto, Toronto General
Hospital, Toronto, Canada
11:40 – 12:00 Questions and Answers with Symposium Panel
10:30 – 11:00 AM Coffee
Available
Grand Atrium
12:15 – 1:30 PM Lunch
Lone Peak Cafe
1:45 – 3:00 PM Young
Investigators Competition
Missouri Ballroom
1:45 – 2:00 PM Growth
Hormone Down-regulation of IL-6 and IL-1β
Induced Hepatic Acute Phase Response is Associated with over Expression of SOCS-3,
Paper 61
Xiaowu Wu, MD, Shriners Burn Hospital, University of Texas
Medical Branch, Galveston, TX
2:00 – 2:15 PM Differential
Effects of MIP-2 and KC on Hemorrhage Induced Neutrophil Priming for Lung
Inflammation, Paper 62
Joanne L. Lomas, MD, Rhode Island Hospital, Brown
University, Providence, RI
2:15 – 2:30 PM Androgens Inhibit Monocyte Cell Signaling, Paper 63
David Gourlay, MD, Harborview Medical Center, University of
Washington, Seattle, WA
2:30 – 2:45 PM NADPH
Oxidase Derived Superoxide is a Key Regulator of Liver Injury After Hemorrhagic
Shock and Resuscitation, Paper 64
Mark Lehnert, MD, University of North Carolina, Chapel Hill,
NC
2:45 – 3:00 PM Mutations
of the Stat 6 Binding Site Have Contrary Effects on IL-6 and IL-10, Paper
65
Mohan Dasu, PhD, University of Texas Medical Branch,
Galveston, TX
3:00 – 3:30 PM Plenary
II: Cutting Edge in Shock Research –
Activated
Missouri Ballroom Protein C and Sepsis
Fletcher B. Taylor, MD, Charles Esmon,
MD, and Gary Kinasewitz, MD,
Oklahoma Medical Research Foundation, Oklahoma City, OK
This session will provide updated clinical trial results and will be followed by descriptions of the protein C network and how and where this network regulates the 1) molecular and cellular processes in vitro and 2) the pathophysiologic processes and stages of E. coli sepsis in vivo. Finally, specific supplemental measures needed to improve efficacy will be reviewed.
3:30 - 5:30 PM Mini-Symposium I: Insights into the Molecular and Cellular Missouri Ballroom Basis of Hemorrhage and Inflammation-Induced
Tissue Injury
Papers 66-75
Co-Moderators:
David Wisner, MD, University of California, Davis Medical Center,
Sacramento, CA & James Cook, MD, University of South Carolina,
Charleston, SC
3:30 – 3:42 PM Vagus
Nerve Stimulation Blunts NF-kβ Activation and Protects Against Hypovolemic
Hemorrhagic Shock, Paper 66
Domenica Altavilla, PhD,
University of Messina Italy & University of Modena and Reggio Emilia
3:42 – 3:54 PM Is
Fluid Regulation During and Following Soft Tissue Trauma and Hemorrhagic Shock
Different Between Males and Proestrus Females? Paper 67
Joachim F. Kuebler, MD, University of Alabama at Birmingham,
Birmingham, AL
3:54 – 4:06 PM Adrenal
Insufficiency as an Occult Source of Shock in the SICU, Paper 68
John McNelis, MD, North Shore-Long Island Jewish Medical
Center, Albert Einstein College of Medicine, New Hyde Park, NY
4:06 – 4:18 PM Gender
Dimorphism in Hepatic Heme Oxygenase Expression
and
Activity After Trauma and Hemorrhagic Shock, Paper 69
Balazs Toth, MD, University of Alabama at Birmingham,
Birmingham, AL
4:18 – 4:30 PM Septic
Shock Causes Net Loss of Heart Mitochondria in Rats, Paper 70
John
A. Watts, PhD
Carolinas
Medical Center, Charlotte, NC
4:30 – 4:42
PM Calcium/Calmodulin-Dependent Kinase II is
Required for
Platelet Activating Factor (PAF) Priming of Inflammatory Cells, Paper 71
Joseph Cuschieri, MD Harborview Medical Center, University of Washington, Seattle, WA
4:42 – 4:54 PM Proinflammatory Lipid in Stored
Blood and From Injury Delay Neutrophil Apoptosis Via MCL-1 Expression,
Paper 72
Rachel L. Carnaggio, MD, Denver Health Medical Center and UCHSC, Denver, CO
4:54 – 5:06 PM The Abdominal Compartment Syndrome (ACS) as a Second Insult During
Systemic PMN Priming Provokes Acute Lung Injury (ALI), Paper 73
Joao B. Rezende Neto, Denver Health
Medical Center, and UCHSC, Denver, CO
5:06 – 5:18 PM L-Selection
Shows Time and Gender Dependency in Association with MODS, Paper 74
Frank Hildebrand, MD, Hanover Medical School, Hanover, Germany
5:18 – 5:30 PM Depressed
IL-12 Production by Monocytes Correlates with a Shift Toward Th2-Type
Lymphocyte Pattern, Duration of SIRS and Adverse Clinical Course After major
Trauma, Paper 75
Zoltan Spolarics, MD, PhD,
UMDNJ, New Jersey Medical School, Newark, NJ
3:30 - 5:30 PM Mini-Symposium II: The
Pathobiology of Inflammatory Cells Amphitheatre During
Experimental Models of Shock, Papers 76-85
Co-Moderators:
Lisa Colleti, MD, University
of Michigan, Ann Arbor, MI and Stephen Jones, PhD, Loyola University,
Maywood, IL
3:30 – 3:42 PM A Role
for Glucocorticoids in Intestinal T Cell Suppression in Alcohol and Burn
Injured Rats, Paper 76
3:42 – 3:54 PM Signaling Defects in Neutrophils During Experimental Sepsis,
3:54 – 4:06 PM Bacterial LPS and Peptidoglycan Induce Synergistic
Production
of TNF-a and Nitric Oxide in Mouse
Macrophage
via Different Mechanisms, Paper
78
David Morrison, PhD
School of Medicine, University of
Missouri, Kansas City, MO
4:06 – 4:18 PM Differential Expression of Trem 1, 2 and 3 In Macrophages
Following
Trauma-Hemorrhage (TH), Paper
79
T.S. Anantha Samy, PhD, University of
Alabama at Birmingham,
Birmingham, AL
4:18 – 4:30 PM IL-6
Knockout Reverses Sepsis-Associated Decreases in Bile Acid Transporter
Expression But Increases Necrosis and Blocks Regeneration, Paper 80
Philip
Kim, MD, Hospital of the University of Pennsylvania, Philadelphia, PA
4:30 – 4:42 PM Selective
Expression of HO-1 By Tissue MФ in Response to
Inflammation: The Role of TLR4 and
Proinflammatory Cytokines,
Paper 81
4:42 – 4:54 PM Novel
LPS-Binding Proteins in Murine Macrophages,
Paper 82,
Nilofer Qureshi, PhD, University of Missouri, Kansas City,
MO
4:54 – 5:06 PM Regulation of Endothelin Signaling By Protein Interactions
After
Endotoxemia, Paper 83
5:06 – 5:18 PM Mesenteric
Lymph From Burned Rats with Evidence of Gut Injury is Cytotoxic for Endothelial
Cells, Paper 84
5:18 – 5:30 PM Sepsis
from Pseudomonas Aeruginosa Pneumonia Induces
Gut Epithelial Cell Cycle Arrest, Paper 85
Craig
M. Coopersmith, MD, Washington University School of Medicine, St. Louis, MO
5:45 - 6:30 PM Young Investigators Reception/Travel Awardees Lamar/Gibbon (Invitation
Only)
6:30 - 7:30 PM Reception
South Patio
7:30 - 9:30 PM Dinner/YIC & Travel Awards Ceremony
Huntley Dining Room (Kevin
Schneider/Speaker)
Topic: “Yellowstone
National Park: Issues and Challenges”
MONDAY, JUNE 10, 2002
6:00
- 7:00 AM Twentieth Annual Presidential Run
Huntley Lodge Lobby
7:00
– 8:00 AM Continential
Breakfast
Lone Peak Café
7:30
- 10:00 AM POSTER SESSION II, Papers 86-150
Mammoth Room
Endotoxin/Sepsis,
Papers 86-109
Gene
Regulation, Papers 110-113
Hemorrhagic
Shock, Papers 114-129
Immunologic
Dysfunction, Papers 130-131
Immunomodulation,
Papers 132-133
Inflammation,
Papers 134-141
Ischemia/Reperfusion,
Papers 142-146
Liver,
Papers 147-150
10:00 – 12:00 SYMPOSIUM
II: Novel Approaches to Current Problems in Missouri Ballroom Critically
Ill Patients
Moderators: Paul Bankey, MD, PhD, University of Rochester, Rochester, NY and Reuven Rabinovici, MD, PhD, Yale University, New Haven,
CT
10:00 – 10:20
AM RNA Editing in Inflammation, Reuven Rabinovici, MD, PhD, Yale
University, New Haven, CT
10:20 – 10:40
AM The Role of Mathematical Models in Predicting the Response
to
Sepsis, Robert
Fulton, MD, University of Louisville,
Louisville, KY
10:40 – 11:00
AM Computer-Based Predictions of the Response to
Trauma/Hemorrhage, Yoram Vodovotz, PhD,
University Pittsburgh, Pittsburgh, PA
11:00 – 11:20
AM Coagulopathy as a Mediator of Organ Dysfunction, Stephen
Lowry, MD, UMDNJ – RWJ Medical School,
New Brunswick, NJ
11:20 – 11:40
AM Nutritional Immunomodulation – has the time come? Paul
Wischmayer, MD, PhD, University of
Colorado, Denver, CO
11:40 – 12:00 Questions
and Answers with Symposium Panel
10:30
– 11:00 AM Coffee
Available
Grand Atrium
12:15 - 1:15PM BUSINESS MEETING
Missouri Ballroom
12:15
- 1:15PM YOUNG INVESTIGATORS RESEARCH FORUM
Amphitheatre Moderated by: Daniel Remick, MD,
University of Michigan,
Ann
Arbor, MI
FREE AFTERNOON
TUESDAY, JUNE 11, 2002
7:00
– 8:00 AM Continental
Breakfast
Lone Peak Café
7:30
- 10:00AM POSTER SESSION III, Papers 151-210
Mammoth Room
Endotoxin/Sepsis, Papers 151-160
Hemorrhagic
Shock, Papers 161-172
Metabolism,
Paper 173
Microcirculation,
Papers 174-177
Monocytes/Macrophages,
Papers 178-183
Multiple
Organ Failure, Papers 184-187
Neural
Aspects, Papers 188-189
Neutrophils,
Papers 190-195
Nitric
Oxide, Papers 196-200
Oxygen
Metabolites, Papers 201-202
Pharmacologic
Agents, Papers 203-205
Pulmonary,
Paper 206
Other, Papers 207-210
10:00 – 12:00 Symposium III:
Pathogenesis, Manifestations and
Current
Missouri Ballroom Therapies of Thermal Injury
Induced Lung Dysfunction
Moderator: Daniel Traber, PhD, University of Texas,
Galveston,TX
Thermal injury both inhalational and burn remain significant
societal problems. Many investigators
within our organization have focused their research and clinical efforts
addressing this problem. This symposium will provide a comprehensive review of
our current understanding of the molecular, cellular and signal transduction
abnormalities induced by thermal injury in the lung. Additional information will be provided both on the role of
nitric oxide in thermal injury induced lung dysfunction and recent advances in
clinical therapies for critically ill patients with both burn/inhalation
injury.
10:00 – 10:20
AM Molecular Mechanisms of Thermal-Injury Induced Acute Lung
Dysfunction
David Greenlagh, MD, University
of California, Davis Medical Center, Sacramento, CA
10:20 – 10:40
AM The Role of Cytokines in Thermal-Injury Induced Acute Lung
Dysfunction
Frank Schmalstieg, MD, PhD, University
of Texas, Galveston, TX
10:40 – 11:00 AM Signal
Transduction Abnormalities During Thermal Injury
Charles Hales, MD, Massachusetts
General Hospital, Harvard Medical School, Boston, MA
11:00 – 11:20
AM The Role of Nitric Oxide in Injury Associated with
Burn/Inhalational
Injury
Glen Warden, MD, University of
Cincinnati, Cincinnati, OH
11:20 –
11:40 AM Pathogenesis – Driven Therapies for
Burn/Inhalational
Induced Acute Lung Injury
William Cioffi, MD, Rhode
Island Hospital, Brown University, Providence, RI
11:40 – 12:00 Questions and Answers with Symposium Panel
10:30 – 11:00 AM Coffee
Available
Grand Atrium
12:15-1:30 PM LUNCH
Lone Peak Café
1:30 - 3:00 PM
WORKSHOP II: Controversies In Shock Outcome
Missouri Ballroom Moderator: Paul Redmond, MD, Cork University Hospital, Cork,
Ireland
A)
Genetic Polymorphism Determines the
Response to
Shock
Pro - Martin Schwacha, PhD, University of Alabama, Birmingham,
AL
Con - Lorrie Langdale, MD, University of
Washington, Seattle,
WA
B) Resuscitation to Maximal vs. Standard
Oxygen
Therapy
During Shock
Pro - Stuart
Myers, MD, PhD, Medical College of Virginia,
Richmond, VA
Con - Greg Timberlake, MD, University of
Mississippi, Jackson,
MS
3:00
- 5:30 PM Mini-Symposium III: Insights into the
Intercellular Mediators
Missouri Ballroom of Shock/Sepsis
Pathophysiology, Papers 211-220
Co-Moderators:
John Marshall, MD, University of Toronto, Ontario, Canada and Wilfried
Lorenz, MD, Unversitat Marburg, Marburg, Germany
3:00 – 3:15 PM Kinetics
of Inducible Nitric Oxide Synthase Expression in a
Neonatal
Rodent Model of Necrotizing Enterocolitis,
Paper 211
Jeffrey
S. Upperman, MD, Children’s Hospital of Pittsburgh, Pittsburgh, PA
3:15 - 3:30 PM The
Role of Nitric Oxide in Cardiac Myocyte Apoptosis
Induced
by Burn Plasma, Paper 212
University of Texas Southwestern Medical Center, Dallas, TX
3:30 – 3:45 PM Source
of Reactive Oxygen Metabolites Has Differential Effects on Enterocyte
Apoptosis, Paper 213
Lawrence
N. Diebel, MD, Wayne State University School of Medicine, Detroit, MI
3:45 – 4:00 PM
Gene Expression Profiles in Mouse
Macrophages in
Response
to CpG DNA, Paper 214
Jianjun Gao, PhD, University of Missouri, Kansas City, Kansas City, MO
4:00 – 4:15 PM Mechanism of the Salutary Effect of 17β-Estradiol Following
Trauma-Hemorrhage: Direct Down Regulation of Kupffer Cell
IL-6
Gene Expression and Release,
Paper 215
Yukohiro Yokoyama, MD, University of Alabama at Birmingham,
Birmingham, AL
4:15 – 4:30 PM Post-Hemorrhagic
Shock Mesenteric Lymph Kills Human
Umbilical
Vein Endothelial Cells Via a Caspase-3 Mediated
Mechanism,
Paper 216
Marson Davidson, MD, UMDNJ, New Jersey Medical School, Newark, NJ
4:30 – 4:45 PM Substance P Primes Human Monocytes Via
Upregulation of
p38
Kinase and Nuclear Factor-kB, Paper 217
Eileen M. Bulger, MD, Harborview Medical Center, University
of Washington, Seattle, WA
4:45 – 5:00 PM
Hemodynamic Alterations and Intestinal
Permeability in
Burn-Injured
Rats Infected with Enterococcus Faecalis
Infection, Paper 218
Masakatsu Goto, MD, Loyola University of Chicago, Maywood,
IL
5:00 – 5:15 PM Glutamine
Enhances Heat Shock Protein 72 Expression and
Preserves High Energy
Phosphate Levels in Lung Tissue Following Sublethal Endotoxin Shock, Paper 219
Kristen Singleton, MD, University of Colorado Health Sciences Center, Denver, CO
5:15 – 5:30 PM Myocardial
UCP2 Expression During Early Septic Shock in the
Rat, Paper 220
Michael Roshon, MD, PhD, Carolinas Medical Center, Charlotte, NC
3:00 – 5:30 PM Mini-Symposium
IV: Advances In the Management of
Amphitheatre Critically
Ill Patients: Bench to Bedside, Papers 221-230
Co-Moderators:
Patricia E. Molina, MD, PhD, HSC, LSU, New Orleans, LA and Timothy
Pruett, MD, University of Virginia, Charlottesville, VA
3:00 – 3:15 PM Sepsis
Gene Expression Profiling Ill: Murine
Circulating Whole Blood Distinguishes Intra-Peritoneal Lipopolysaccharide (LPS)
From Cecal Ligation and Puncture (CLP), Paper 221
T.
Philip Chung, MD, Washington University School of Medicine, St. Louis, MO
3:15 – 3:30 PM Isolation
of a Novel Gene that is Expressed Differentially in the Bone Marrow Cells
Following Thermal Injury and Sepsis, Paper 222
Asif Daud, MD, Loyola University Medical Center, Maywood, IL
3:30 – 3:45 PM Gene Expression Profiles of Human Epithelial Cells in
Response
to Heat Characterized Using High-Density
Oligonucleotide
Microarrays, Paper 223
Jason Laramie, MD, Washington
University School of Medicine,
St. Louis, MO
3:45 – 4:00 PM Effects of Ciglitazone, A Peroxisome Proliferator-Activated
Receptor-Gamma
(PPARγ) Ligand, In Experimental Sepsis
Paper 224
Maeve Sheehan, MD, Children’s Hospital
Medical Center,
University of Cincinnati, Cincinnati,
OH
4:00 – 4:15 PM Substance P Upregulation of Nitric Oxide Synthase is
Enhanced
by EGF Receptor Inhibition,
Paper 225
Joanne P. Anthony, MD, Harborview
Medical Center, University of
Washington, Seattle, WA
4:15 – 4:30 PM Partial Liquid Ventilation (PLV) Decreases Serum Level of
Lipid
Mediators in Experimental Acute Lung Injury. Paper 226
Yoh Hirayama, MD, Graduate School of
Medicine, Chiba
University, Chiba, Japan
4:30 – 4:45 PM Recovery of Adenosine Deaminase (ADA) Activity After
Inhibition
with Pentostatin During Sepsis, Paper
227
William R. Law, PhD, University of
Illinois College of Medicine,
Chicago, IL
4:45 – 5:00 PM Perioperative Administration of Polymyxin B Shortens
Hospital
Stay in Surgical Patients,
Paper 228
Toshikiko Mayumi, MD, Nagoya University
School of Medicine,
Nagoya, Japan
5:00 – 5:15 PM Norepinephrine Influences the Clonogenic Potential of Bone
Marrow
Early and Late Progenitors Following Thermal Injury
and
Sepsis, Paper 229
Stephen B. Jones, MD Loyola University
Medical Center,
Maywood, IL
5:15 – 5:30 PM Evidence of Alveolar Recruitment Manifested as “Steps” in
the
Whole Lung Pressure-Volume Curve,
Paper 230
Jeffrey M. Halter, MD, SUNY Upstate
Medical University,
Syracuse, NY
6:30 – 7:30 PM Reception
Grand Atrium
7:30 – 9:30 PM Dinner/Scientific
Achievement and Service Awards Ceremony
Missouri Ballroom